An Economic Method to Identify Cultivars and Elite Lines in Radish (Raphanus sativus L.) for Small Seed Companies and Independent Breeders

Radish is a root vegetable crop, and many F1 cultivars have been developed by exploiting heterosis. Considerable time resources are required to test the performance combining ability of parental lines; therefore, economical methods identify them needed before field tests. This study was conducted determine minimum number markers elite lines. PCR-based were applied radish lines selected based on chromosomal location, polymorphic information content, allele frequency. The power marker sets detect genetically similar or confirmed using with high genetic similarity same parents. Eleven successfully identified not only all 105 tested but also reported in previous studies. Fourteen markers, including nine among 11 for cultivar identification, distinguished 115 tested, ones parents separated at F2–F6 generations. identification require 14 respectively, simple PCR gel electrophoresis instruments. method very can be easily other crops.